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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: J Immunol. 2012 Mar 5;188(7):3053–3061. doi: 10.4049/jimmunol.1102414

Fig. 2. Comparison of αGC with αGC acC8 in vivo.

Fig. 2

B6 mice were injected intraperitoneally with 2μg αGC or 5μg αGC acC8 unless otherwise indicated. (A) Serum IL-4 at 2 h and serum IFNγ at 24 h. (B) Serum IL-12p70 at 6 h. (C) Splenic TCRβ+ CD1d-αGalCer+ NKT cells collected at 1 h, 2 h and 6 h as indicated and directly stained for intracellular IL-4 and IFNγ. Results compiled from 3 separate experiments (D) Spleen cells collected at 6 h, stained with anti-DX5 and anti-IFNγ and displayed after gating on TCRβ−B220−cells. Left, representative FACS plots; right, summary figures showing the frequency of IFNγ secreting cells among NK cells and their MFI for IFNγ. Results compiled from 3 separate experiments. (E) Mice received 5 injections (at 0, 1, 2, 3 and 5 h) of 5μg or 1 injection of 25μg of αGC acC8. Sera were collected at 6h for IL-12p70, 24 h for IFNγ and 2h for IL-4 and were compared with sera of mice receiving 1 injection of 2μg αGC at 0 h. Results compiled from 2 separate experiments.