Figure 1. Schematic of the modified Stamper-Woodruff Assay.

Cells to be tested for L-selectin ligand activity are placed within a defined region of the glass slide by cytocentrifugation. All other steps, including fixation (typically with glutaraldehyde), are performed similarly to conventional Stamper-Woodruff assays (which utilize frozen sections of tissue, e.g., lymph node). Shear stress is created by rotation of the slide platform at 80 rpm.