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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Immunol. 2012 Feb 24;188(7):3447–3457. doi: 10.4049/jimmunol.1102211

Figure 2. Ligation of β2 integrins by CD11b antibodies inhibits IFN-α signaling.

Figure 2

Primary human macrophages were plated onto control IgG2b coated wells or anti-CD11b coated wells.

A, Macrophages were plated for 2 h and then IFN-α (50 U/mL) was added for an additional 2 h to assess induction of IFN-α response genes. mRNA expression was measured by qPCR, and results are presented as mean ± SD of triplicate wells normalized relative to GAPDH mRNA. Data are representative of 3 independent experiments.

B, Macrophages were plated for 4 hr and then IFN-α (500 U/mL) was added for an additional 15 min to assess phosphorylation of STAT1. Whole cell lysates were immunoblotted with Abs against phospho-STAT1 (Y701) and STAT1. Data are representative of 3 independent experiments.