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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Immunol. 2012 Feb 24;188(7):3447–3457. doi: 10.4049/jimmunol.1102211

Figure 3. β2 integrin inhibition of IFN-α signaling is mediated by a p38 and ERK-dependent pathway.

Figure 3

Primary human macrophages were pretreated with either the vehicle control DMSO or two MAPK inhibitors: p38 inhibitor SB203580 (15 μM) and MEK inhibitor U0126 (15 μM) for 30 min. Macrophages were then plated onto control wells or fibrinogen (Fb) coated wells, and IFN-α (1000 U/mL) was added for an additional 2 h to assess induction of IFN-α response genes (A) or 15 min to assess phosphorylation of STAT1 (B).

A, Macrophages were plated for 2 h prior to addition of IFN-α. mRNA expression was measured by qPCR, and results are presented as mean ± SD of triplicate wells normalized relative to GAPDH mRNA. Data are representative of at least 3 independent experiments.

B, Macrophages were plated for 4 h prior to addition of IFN-α. Whole cell lysates were immunoblotted with Abs against phospho-STAT1 (Y701) and STAT1. Data are representative of 2 independent experiments.