Skip to main content
. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Immunol. 2012 Feb 24;188(7):3447–3457. doi: 10.4049/jimmunol.1102211

Figure 5. Concurrent ligation of ITAM-coupled receptors and TLR4 inhibits induction of the TLR4-induced IFN-β-mediated autocrine loop.

Figure 5

Primary human macrophages were added to control or fibrinogen (Fb) coated wells with or without LPS (10ng/mL) (A–C). White bars represent unstimulated controls cells at each timepoint. mRNA expression was measured by qPCR, and results are presented as mean ± SD of triplicate wells normalized relative to GAPDH mRNA.

A, Expression of TNF, IL1B and IL10 mRNAs was measured. Data are representative of 3 independent experiments.

B, Induction of autocrine IFN-β mRNA expression was measured by qPCR, and IFN-β protein from culture supernatants was measured by ELISA. Data are representative of 3 independent experiments.

C, Induction of type I IFN dependent CXCL9 and CXCL10 mRNAs downstream of LPS stimulation was measured. Data are representative of 3 independent experiments.

D, Cells were treated with zymosan (100μg/mL), LPS (10 ng/mL), or zymosan plus LPS. Induction of LPS-dependent and type I IFN dependent mRNAs were measured. Data are representative of 3 independent experiments.