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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Immunol. 2012 Feb 24;188(7):3447–3457. doi: 10.4049/jimmunol.1102211

Figure 6. Primary human Mφ pre-ligated with fibrinogen are protected from L. monocytogenes-induced apoptosis.

Figure 6

Primary human macrophages were plated onto control wells or fibrinogen (Fb) coated wells for 18 h. Culture media was changed, and cells were infected with live L. monocytogenes (wild-type strain 10403S) at MOI 10, 20 (A) or MOI 5 (B, C).

A, Flow cytometry was used to assess apoptosis, measured by annexin V binding to phosphatidylserine in the DAPI negative population. In vitro infections were performed in triplicate wells, and results are presented as mean frequency ± SD of triplicate wells. Cell death was measured by positive staining for both annexin V and DAPI. Data are representative of at least 3 independent experiments.

B, Viable bacteria from macrophages were quantified 4 h post infection. 4 independent experiments are shown, with each symbol representing a different donor. Statistical analysis was done using the 2-tailed, paired Student t test, and intracellular burden between control-infected and Fb-infected macrophages was not statistically different.

C, Induction of multiple infection induced mRNAs was measured by qPCR, and results are presented as mean ± SD of triplicate wells normalized relative to GAPDH mRNA. Data are representative of at least 3 independent experiments.