Skip to main content
. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Immunol. 2012 Feb 22;188(7):3294–3306. doi: 10.4049/jimmunol.1101111

FIGURE 3. TCDD inhibits endogenous Ig heavy- and light-chain genes.

FIGURE 3

CH12.LX cells (2.5 × 104 cells/mL) stimulated with LPS and treated with 10 nM TCDD or the vehicle control (0.01% DMSO, denoted 0 nM TCDD) were plated into 12-well plates and incubated for 24, 36, or 48 h prior to total RNA isolation. Total RNA (200 ng) was reverse transcribed to cDNA and utilized to amplify the endogenous heavy- and light-chain genes (Cα and Cκ respectively) via SYBR®Green real-time PCR. The results are expressed as the relative quantification (RQ) value compared to cells cultured with media alone (NA, naive). Results are representative of three separate experiments (n=3 for each treatment group). Statistical differences compared to the respective vehicle control were determined by a 1-way ANOVA with a Dunnett’s post-hoc test; ** P < 0.01 or *** P<0.001.