Figure 4.
Effects of AM6-36 on the expression of caspases. (A) HL-60 cells were treated for 24 h with various concentrations of AM6-36 and protein expression was measured by Western blot analysis. (B) HL-60 cells were treated with serially diluted AM6-36 for 24 h and then incubated with the substrates of caspase-3/-7, caspase-8, or caspase-9. The fold induction in comparison with vehicle (DMSO)-treated control is demonstrated as a bar graph. *P value less than 0.05 is considered statistically significant from control group (white bars) at each time and caspase.