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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Immunol. 2012 Feb 29;188(7):3496–3505. doi: 10.4049/jimmunol.1103182

FIGURE 1.

FIGURE 1

SLFs up-regulate CXCL2 in response to NTHi. A, Migration assays show that CXCR2 deficiency inhibits migration of PMNs in response to the NTHi-induced SLF-derived molecules. WT: wild-type PMNs, Cxcr2-/-: CXCR2-deficient PMNs, NTHi-: conditioned medium from the NTHi-unexposed RSL cells, NTHi+: conditioned medium from the NTHi-exposed RSL cells. B, ELISA analysis shows that the RSL cells up-regulate CXCL2 in response to NTHi. ND: not detected. The experiments were performed in triplicate and repeated twice. Values are given as the mean ± standard deviation (n = 3). *: p < 0.05. C, Immunolabeling shows that CXCL2 is highly expressed in the spiral ligament of the NTHi-inoculated mice (NTHi), but not in the saline-inoculated mice (Con). Original magnification: x50.