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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Immunol. 2012 Feb 29;188(7):3496–3505. doi: 10.4049/jimmunol.1103182

FIGURE 2.

FIGURE 2

Activation of the c-Jun is required for NTHi-induced CXCL2 up-regulation. A, Transcription factor ELISAs show that the SLFs activate c-Jun in response to NTHi. B, Phosphorylation assays show that c-Jun of the nuclear fraction is phosphorylated in response to NTHi. C, NTHi-induced CXCL2 up-regulation is noted to be inhibited by Tanshinone IIA in a dose-dependent manner. D, Luciferase assays demonstrate that a dominant-negative inhibition of c-Jun (TAM 67) suppresses NTHi-induced CXCL2 up-regulation. E, ELISA analysis shows that a dominant-negative inhibition of c-Jun (TAM 67) markedly suppresses NTHi-induced CXCL2 up-regulation. ND: not detected. pcDNA: a mock transfection. Results were expressed as fold-induction, taking the value of the non-treated group as 1. The experiments were performed in triplicate and repeated twice. Values are given as the mean ± standard deviation (n = 3). *: p < 0.05.