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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Immunol. 2012 Feb 29;188(7):3496–3505. doi: 10.4049/jimmunol.1103182

FIGURE 4.

FIGURE 4

The AP-1 motif is critically involved in NTHi-induced CXCL2 up-regulation. A, Luciferase assays using nested deletions show that the NTHi-responsive element is located between -564 and -134 of the rat CXCL2 promoter region. B, Luciferase assays with site-directed mutagenesis show that the mutation of the proximal AP-1 motif inhibits NTHi-induced CXCL2 up-regulation more than that of the distal AP-1 motif. Mutation of both AP-1 motifs is noted to completely inhibit NTHi-induced CXCL2 up-regulation. Results were expressed as fold-induction, taking the value of the non-treated group as 1. The experiments were performed in triplicate and repeated twice. Values are given as the mean ± standard deviation (n = 3). *: p < 0.05.