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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Immunol. 2012 Mar 2;188(7):2977–2980. doi: 10.4049/jimmunol.1100830

Figure 3. Alendronate requires mast cells to induce peritonitis.

Figure 3

A) WT C57BL/6 (n=6/group), Rag1−/−(n=7/group), beige (BgJ, n=5–6/group) or Pretty2 (n=3/group) mice were injected with 0.8 mg ALD (black bar) or vehicle (white bar). The peritoneal lavage was collected after 3 days. B) W/Wv mice, littermate WT mice, and W/Wv mice reconstituted BMMC received ALD (black) or vehicle (white). The peritoneal lavage was collected and total cell number was determined. C) ALD was injected in WT mice (n=10–12/group) treated with cromolyn (Cromo, n=7–8/group) or compound 48/80 (48/80, n=5/group). Peritoneal infiltrating cells were collected 3 days after ALD (black) or vehicle (white) injection.D)Degranulation of BMMC treated with 100μM each compound for 1.5h measured by β-hexosaminidase released in supernatant (Å405). For all panels data is the pooled means+SEM from 2–3 independent experiments, *p<0.05 by one-way ANOVA with Dunnett's test.