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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Immunol. 2012 Feb 24;188(7):3416–3425. doi: 10.4049/jimmunol.1102746

Figure 2.

Figure 2

The alternative pathway of complement and properdin is required for killing of C4BP-binding strains of N. gonorrhoeae by mAb 2C7. A. Survival of gonococcal strains was measured in serum bactericidal assays in the presence of NHS alone, NHS plus mAb 2C7, or NHS plus mAb 2C7 where either factor B or properdin function was blocked with anti-factor Bb (α-Bb) or anti-properdin (α-P), respectively. Each data point represents the mean (±SD) of at least 2 independently repeated experiments. *, p<0.001 (2-tailed Student’s t-test) compared to the corresponding survival seen with mAb 2C7+NHS. B. C4BP binding to N. gonorrhoeae strains. N. gonorrhoeae strains 442089, 24-1 (LOS sialylated by growth in media containing CMP-NANA; labeled 24-1 sia+), FA1090, 15253, and 252 were incubated with 1%, 3% or 10% NHS and C4BP bound to the bacteria surface was measured by flow cytometry. One representative experiment of 3 independently performed experiments is shown. Numbers represent the average of the median fluorescence of each of the three experiments.