Skip to main content
. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Immunol. 2012 Mar 5;188(7):3071–3079. doi: 10.4049/jimmunol.1103554

Figure 2.

Figure 2

Expansion of Q9:VP2.139-specific CD8 T cells depends on CD28 and CD40L costimulation. A cocktail of MR-1 (α-CD40L) and CTLA-4-Ig were administered to B6.Kb−/−Db−/− mice coincident with MPyV inoculation and then once weekly for 1 mo. Control mice received no Ab. A. Frequency (± SD) of CD8+ Q9:VP2.139-specific cells in blood over time, determined by cell surface Q9:VP2.139 tetramer staining. B. Number (± SD) of splenic Q9:VP2.139-specific CD8 T cells at d 28 p.i. was determined by tetramer staining and VP2.139 peptide-stimulated intracellular IFN-γ production. C. Numbers of MPyV genome copies were determined by qPCR in indicated organs at d 28 p.i. Each point corresponds to an individual mouse, and horizontal lines indicate geometric mean. Data are representative of six mice per group pooled from two independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.