Figure 1. MPO activity and LTB₄ production in the lungs is dependent on CXCL1 during K. pneumoniae infection.

MPO activity (A) and LTB₄ levels (B) in homogenized (unlavaged) whole lungs of WT (C57Bl/6) and CXCL1^-/- mice infected with K. pneumoniae (10³ CFU/mouse) for 24 and 48 h. Data are presented as means ± SEM. n=6-8 mice/group. (* indicates p<0.05 compared with CXCL1^-/- mice). The levels of p67^phox, p47^phox, and iNOS (C) in K. pneumoniae-infected homogenized whole lungs of WT and CXCL1^-/- mice at 24 and 48 h post-infection. The blot is representative of three individual blots with identical results. Densitometric analysis of p67^phox, p47^phox and iNOS expression (D) in homogenized lungs. Densitometry was performed from three separate blots. Data are expressed as means ± SEM. n=6-8 mice/group. (* indicates p<0.05 compared to CXCL1^-/- mice). Levels of p67^phox and p47^phox in cytosolic and membrane fractions of the lung (E) upon K. pneumoniae infection. This is a representative blot of three independent experiments. GAPDH is a cytosolic marker, pan-cadherin is a plasma membrane marker. Densitometric analysis of p67^phox and p47^phox levels (F) in cytosol and membrane fractions of the lung following K. pneumoniae infection. Protein expression was quantitated from three separate blots. Data are shown as means ± SEM. For experiments E-F, a total of 6-8 mice were used in each group. (* indicates p<0.05 as compared to CXCL1^-/- mice.)