Figure 3. CXCL1 is essential for Klebsiella-induced expression of p67^phox, p47^phox, and ROS/RNS production by neutrophils.

Levels of p67^phox and p47^phox (A) in bone marrow-derived neutrophils from WT and CXCL1^-/- mice after infection with K. pneumoniae (MOI of 1). This is a representative blot from three separate experiments. Densitometric analysis from three separate blots (B) shows the expression of p67^phox and p47^phox in K. pneumoniae stimulated-neutrophils that were normalized against GAPDH. (* indicates p<0.05 as compared to CXCL1^-/- neutrophils). MPO activity and nitrite and H₂O₂ release (C) in WT and CXCL1^-/- neutrophils stimulated with K. pneumoniae. The levels of MPO, nitrite, and H₂O₂ were measured in infected neutrophils at 30, 60, and 180 min post-infection. Experiments were performed in triplicates. Bacterial killing capacity (D) of K. pneumoniae-infected neutrophils from WT and CXCL1^-/- deficient mice was determined by assessing extracellular and intracellular CFUs at 30, 60, and 180 mins post-infection with K. pneumoniae (MOI of 1). Relative phagocytosis (E) of K. pneumoniae-infected WT and CXCL1^-/- neutrophils at 30 min post-treatment (MOI of 1). For experiments A-E, a total of 5-8 mice/group were used. (* indicates p<0.05 compared with CXCL1^-/- neutrophils).