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F. tularensis LVS inhibits Fas-induced apoptosis. Neutrophils were preincubated with LVS (MOI 200:1) for 1 h, and then treated with 500 ng/ml anti-Fas IgM for an additional 6 h. Apoptosis was measured using Annexin V staining and flow cytometry. Data indicate the percentage of Annexin V-positive cells and are the mean ± SEM (n=3).* P <0.05.
