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pH-Dependence of the observed hyperfine splitting constant, aN, of the R-SG spin probe. EPR spectra were acquired at 37 °C. The solid line is the fit of experimental data with standard titration curve yielding pKa=6.6, aN(RH+)=14.24 G and aN(R)=15.27 G. Insert: chemical structure of the R-SG spin probe.
