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. Author manuscript; available in PMC: 2013 Apr 20.
Published in final edited form as: J Mol Biol. 2012 Feb 14;418(1-2):32–46. doi: 10.1016/j.jmb.2012.02.013

Figure 6.

Figure 6

Kinetics of UvrD monomer translocation monitored by the arrival of UvrD at the 5′-end of 5′-F-(dT)L in the presence of different molar ratios of ADP:ATP. UvrD is pre-incubated with excess 5′-F-dTL in T20 buffer then rapidly mixed with ATP, ADP, MgCl2, and heparin in T20 buffer at 25°C to initiate translocation. (A)- Translocation time courses at 1:1 and 2:1 ADP:ATP concentration ratios at a constant ATP concentration of 500 μM ATP. (B)- Translocation time courses at 2:1 ADP:ATP concentration ratio at constant ATP concentration of 25 μM ATP. The smooth black curves are simulations using eq. (2) and the best-fit parameters determined from a combined global NLLS analysis of the time courses (Table 2). (C)- The observed translocation kinetic step size as a function of ADP:ATP ratio. The kinetic step size was determined as in Figure S2A.