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. 2011 Jul 20;11(1):132–144. doi: 10.1007/s12311-011-0297-7

Table 1.

Details of the stereologic analysis procedures

P
Obj. 1 sla-x, sla-y [μm] OGL ML IGL WM Total
Volumes 1.25× 500 9.681 13.839 25.152 9.100 57.772
Obj. 2 sln-x, sln-y [μm] a [μm2] h [μm] d [μm] ∑OD N t [μm] CEpred.[n]
GCs 20× 1,700 10 10 4 224 699 8.8 0.038
PCs 20× 500 60 60 4 394 466 12.9 0.046

OGL outer granular cell layer, ML molecular layer, IGL inner granular cell layer, WM white matter, Obj. 1 objective used for delineating the regions of interest and point counting, sla-x and sla-y, distance between the points used for volume estimates in mutually orthogonal directions x and y, ∑P average number of points counted, GCs granule cells, PCs Purkinje cells, Obj. 2 objective used for counting neurons, sln-x and sln-y distance between the unbiased virtual counting spaces used for counting neurons in mutually orthogonal directions x and y; a and h base and height of the unbiased virtual counting spaces, d depth within the section at which the unbiased virtual counting spaces were placed, ∑OD average number of unbiased virtual counting spaces used, ∑N average number of neurons counted, t measured actual average section thickness of the sections after histological processing, CE pred. [n] average predicted coefficient of error of the estimated total neuron numbers using the prediction method described by [44]