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. 2011 Jan 19;31(3):979–991. doi: 10.1523/JNEUROSCI.4960-10.2011

Figure 2.

Figure 2.

Depolarization-induced presynaptic silencing is not dependent on increases in intracellular free calcium from any source. A, Representative examples of sucrose-evoked EPSCs and action potential-evoked EPSCs elicited in autaptic neurons after 4 h treatment in normal calcium-containing media (control) or calcium-free media supplemented with 20 μm of the cell-permeant fast calcium chelator BAPTA-AM. B, Summary of results for sucrose-evoked EPSC charge and action-potential-evoked EPSC amplitude after treatment with control or with calcium-free, BAPTA-AM-supplemented extracellular media in sister cultures (n = 9–11 neurons). *p < 0.05. C, Example fields of neurons filled with the calcium indicator fluo-4 before (0 mm) and after (0.5 mm) perfusion of CaCl2. Neurons were pretreated for 4 h with 30 mm KCl in either calcium-containing media (depolarized) or calcium-free media supplemented with 20 μm BAPTA-AM (depolarized BAPTA-AM). Scale bar, 50 μm. D, Representative examples of sucrose-evoked EPSCs in autaptic neurons after 4 h treatment in calcium-free media supplemented with 20 μm BAPTA-AM and either 30 mm NaCl (baseline) or 30 mm KCl (depolarized).