Table 1.

Reaction Conditions of Each Step of Quantitative Real-Time Polymerase Chain Reaction (PCR)

Step	Duration, seconds	Temperature, °C	Cycles, n
Initial denaturation	600	95	1
Denaturation	30	95	40
Annealing	60	1–5 below Tm	40
Extension	60	72	40

Tm, melting temperature.