FIG. 6.

Compares the images of confocal microscopy with and without addition of TGF-β. Greater extracellular matrix deposition is observed when TGF-β is added compared with the control groups. DAPI staining of nuclei in the construct shows that the number of cells at the surface of the mesh increased progressively in TGF-β groups, eventually formed a thicker tissue around the mesh; (A) control group from top view, (B) control group from side view; (C) TGF-β group from top view; and (D) TGF-β group from side view. Nuclei, fibroblasts, smooth muscle cells, and endothelial cells were stained as blue, green, red, and magenta, respectively. TGF-β, transforming growth factor.