FIG. 7.

To determine the structure of a full construct without formation of the vermillion, a preliminary experiment was performed in which the cells were not allowed to migrate and come into contact, being kept separated over the Alloderm^® when submersed and using 1.2 mM calcium medium to prevent cell migration. In this case, alveolar ridge cells were used in the oral area. In (A) a panoramic view of the full 3D lip construct is shown, with skin seeded cells on the left and alveolar ridge cells on the right. Cells did not migrate toward the center and the barrier area remained without cells. A magnified view of the structure and keratin 10 expression of both epithelial and oral areas is shown in (B) and (C) respectively. This experiment illustrates the possibilities of the technique in the formation of 3D muco-cutaneous constructs, where varied cell types can be simultaneously seeded, or not, in the different compartments formed by the barrier. Color images available online at www.liebertonline.com/tec