| TaqMan Universal PCR Master Mix (2×) | 12.5 μl |
| 60 ng/μl Forward primer | 1.5 μl |
| 60 ng/μl Reverse primer | 1.5 μl |
| 20 pmol/μl TaqMan probe | 0.25 μl |
| ddH2O | 6.25 μl |
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