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. Author manuscript; available in PMC: 2012 Mar 24.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Sep 2;389(3):455–460. doi: 10.1016/j.bbrc.2009.08.165

Figure 4. Interaction between PrLZ and 14-3-3 proteins as detected with mammalian two-hybrid assay.

Figure 4

A, the assay was calibrated with empty vectors of the two-hybrid system and with kit controls (pACT-MyoD and pBIND-Id). B, results of the PrLZ isoforms in pACT co-transfected with each 14-3-3 cDNA in pBIND. TPD52, the prototype of the TPD52 family, was included in the study. Similar results were obtained in a complementary experiment, when PrLZ isoforms in pBIND was examined for interaction with 14-3-3 in pACT (data not shown). When transfected individually, neither the PrLZ nor 14-3-3 constructs induced significant luciferase activity from the pG5-luc reporter (data not shown). Each data represent the mean of triplicate assays. Standard deviation was less than 5% of the mean and is not shown.