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. 2012 Feb 15;125(4):831–843. doi: 10.1242/jcs.084301

Fig. 6.

Fig. 6.

Arterial shear stress protects ECs against apoptosis. (A) Morphology of serum-starved HUVECs after 72 hours exposure to static (left) and shear stress (right) conditions. White arrow indicates EC alignment in the direction of flow. Scale bars: 100 μm. (B) Flow cytometric analysis of apoptosis following annexin V and PI staining of HUVECs grown under static and shear stress conditions. A representative set of scatter dot plots (top) shows the percentage of early and late stage apoptosis of static and shear-stress-induced HUVECs. Exposure to shear stress led to a significant decrease in the percentage of apoptotic cells, as quantified in the bar graph (bottom). EC apoptosis is expressed as means ± s.e.m. (n=3), *P<0.05.