Fig. 6.

Reduced colocalization of Cx43 with N-cadherin in the ICD of αT-catenin KO heart. Adult cardiomyocytes (n=10) isolated from either WT or αT-catenin KO mice were co-stained for Cx43 (red) and N-cadherin (green). Boxed regions in the merged images are also shown at higher magnification. The images were analyzed by using ImageJ software with a Pearson–Spearman correlation colocalization plug-in to perform quantitative statistical colocalization on the two-color confocal images, as described previously (French et al., 2008). (A) In images of WT cells, strong positive correlations for colocalization between Cx43 and N-cadherin in the ICD were indicated by an rp value of 0.45 and an rs value of 0.49. (B) In images of αT-catenin KO cells, reduced colocalization between Cx43 and N-cadherin in the ICD was indicated by an rp value of 0.20 and an rs value of 0.25. Scale bars: 50 μm.