. 2011 Sep 25;6(1):10–15. doi: 10.1007/s12105-011-0302-y

Table 1.

Clone, pretreatment for epitope demasking, dilution, and incubation times for primary antibodies

Antibody	Clone	Pretreatment	Dilution	Incubation time
Ang-1^a	Polyclonal	Citrate 0,01 M; pH 6.0; 95°C; 30mim	1:500	60 min
Ang-2^a	Polyclonal	Citrate 0,01 M; pH 6.0; 95°C; 30mim	1:500	60 min
Tie-2^a	Monoclonal	Tris–EDTA; pH 9.0; 95°C; 30mim	1:50	Overnight
VEGF^b	Monoclonal	Citrate 0,01 M; pH 6.0; 95°C; 30mim	1:100	Overnight
α-SMA^c	A4		1:300	60 min

^aAbcam Inc., Cambridge, MA

^bSanta Cruz Biotechnology Inc., Europe

^cDako cytomation, Glostrup, Demark A/S