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Dose response curves of PI3K inhibitors LY294002 and wortmannin. A. IC50 values of LY294002 and wortmannin were measured with FL-PIP2 as the PI3K substrate; B. IC50 values of LY294002 and wortmannin were measured with BODIPY-PIP2 as the PI3K substrate. Each experiment was carried out in duplicate and repeated three times.
