Skip to main content
. Author manuscript; available in PMC: 2012 Mar 25.
Published in final edited form as: ACS Chem Biol. 2011 Jan 10;6(3):223–228. doi: 10.1021/cb100308n

Figure 4.

Figure 4

WH-15 reports PLC enzymatic activity in cell lysates. (A) Real-time fluorescence of WH-15 (50 μM) cleavage catalyzed by PLC isozymes in cell lysates and normalized to the initial fluorescence of the reaction with lysates derived from cells transfected with empty vector. (B) HEK-293T cells were lysed and western blotting was performed for the indicated PLC isozymes.