Expression of Ptger4 in the ductus arteriosus. (A, B) Transverse sections of DA from the 18.5 day mouse fetus; (C, D) longitudinal sections of DA from neonate; (E, F) transverse sections of neonatal aorta (6 h after birth); (G, H) transverse section of neonatal pulmonary trunk; sections were hybridized with 35S labeled Ptger4 antisense probe (A,C,E,G) or Ptger4 sense probe (B,D, F,H). Scale bar is 150µm. Black arrowhead points to junction between the SMC of the DA and the aorta; white arrowheads depict Ptger4 expression in endothelial cells. I) Ptger4 transcript levels in abdominal aorta, aortic arch, and DA as measured by qPCR