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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2012 Feb 11;97(0):109–119. doi: 10.1016/j.prostaglandins.2012.02.001

Figure 5.

Figure 5

Cell lineage restricted loss of EP4R expression. Expression of Ptger4 was examined by qPCR in (A) aortic arch (n=8), (B and D) kidney (n=6), and (C) DA (n=5) isolated from animals (E18.5) heterozygous for Ptger4 null, Ptger4flox-neo, and genetically identical littermates expressing either Tek-Cre (A and B) or Tgln-Cre (C and D). Gene expression was normalized to either GAPDH (A, B, D) or β-2 microglobulin (B2M) (C). Ptger4 levels are decreased by approximately 80% in the aorta of mice expressing the endothelial specific Cre, while no significant change is observed in the kidney. While the majority of Ptger4 expression by the DA is lost in tissue expressing Cre in the SMC, the expression remains higher than that detected in DA from Ptger4−/− mice, reflecting the continued expression of Ptger4 by endothelial cells. . Statistical analysis: ** p < 0.01, *** p<0.001.