Table 1.
Survival of mice after cell lineage specific disruption of the Ptger4 floxed allele.
| Tissue Specific |
Breeding | Age, Days |
n | n Cre+ |
Ptger4 Genotype |
Survival Observed / Expected |
p value observed vs expected |
|---|---|---|---|---|---|---|---|
| Smooth Muscle (Tagln-Cre) | TaglnCre/+ Ptger4+/− X Ptger4f/f | P0 | 163 | 72 | f/− | 20/48 | < 0.001 |
| P3 | 143 | 54 | f/− | 2/48 | < 0.001 | ||
| f/+ | 52/48 | - | |||||
| TaglnCre/Cre Ptger4+/− X Ptger4f/f | P0 | 122 | 122 | f/− | 56/66 | - | |
| P3 | 66 | 66 | f/− | 0/66 | < 0.001 | ||
| f/+ | 66/66 | - | |||||
| TaglnCre/+ Ptger4+/− X Ptger4f/+ | P0 | 207 | 119 | f/− | 20/27 | - | |
| P3 | 187 | 99 | f/− | 0/27 | < 0.001 | ||
| +/+ | 33/27 | - | |||||
| +/− | 27/27 | - | |||||
| f/+ | 39/27 | - | |||||
| Neural Crest (Wnt1-Cre) | Wnt1Cre/+ Ptger4+/− X Ptger4f/f | P0 | 249 | 126 | f/− | 56/64 | - |
| P3 | 193 | 70 | f/− | 0/64 | < 0.001 | ||
| f/+ | 70/64 | - | |||||
| Endothelium (Tek-Cre) | TekCre/+ Ptger4+/− X Ptger4f/f | P0 | 285 | 139 | f/− | 60/75 | - |
| P3 | 278 | 132 | f/− | 53/75 | 0.0267 | ||
| f/+ | 79/75 | - | |||||
n = total numbers of pups found after birth. n Cre+ = number of pups with Cre transgene.
Expected # is the Mendelian expectation of Ptger4flox/− Cre+ pups calculated from the numbers of pups observed with at least one intact copy of the Ptger4 locus (wild type or ptger4flox in the absence of Cre). f = floxed locus. P-value calculated with proportion test.