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. Author manuscript; available in PMC: 2012 Jul 22.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Jun 23;411(1):115–120. doi: 10.1016/j.bbrc.2011.06.107

Fig. 3.

Fig. 3

RUNX1 regulates SERPINB13 expression. (A) Immunoblotting for RUNX1 from HaCaT cells stably expressing control or RUNX1 shRNA or vector control (−). Ponceau S staining shows the relative protein loading. Arrows indi cate RUNX1 isoforms decreased by shRNA. (B) Quantitative RT-PCR analysis of SERPINB13 mRNA using RNA prepared from 3 independent vector control (−) pools, or 3 control or RUNX1 shRNA pools. The relative expression was calculated after correcting for cDNA input using human GAPDH primers. Error bars indicate the differences for data averaged from 3 pools. (C) HA immunoblot from HaCaT stably expressing HA-RUNX1 or vector control. (D) Quantitative RT-PCR analysis of SERPINB13 mRNA using RNA prepared from 3 independent vector control pools or 3 HA-RUNX1 expressing pools. The fold repression was calculated after correcting for cDNA input as described in B.