Figure 5.

Histone modifications at exon-specific bdnf promoters during memory consolidation. A, At the bdnf gene, PH3/AcH3 and AcH3 levels were increased at promoter 4 during fear memory consolidation relative to naive controls (PH3/AcH3, t₍₂₎ = 4.185, p = 0.05; AcH3, t₍₂₎ = 6.21, p = 0.0250, n = 3). AcH4 levels were significantly reduced at promoter 2 relative to naive controls (t₍₂₎ = 12.26, p = 0.0066, n = 3). The top shows the schematic location of the bdnf promoters preceding each exon (P1, P2, P4, and P6) as indicated by the star. In the graph, the areas with no bars indicate that the anti-histone antibody precipitated negligible levels of the bdnf promoter regions that were not detectable. B, At the bdnf promoter 4, AcH3 levels were increased during memory consolidation and significantly attenuated with DNMT inhibition [zebularine (ZEB)] relative to naive controls (t₍₈₎ = 3.047, p = 0.0318, n = 4–6). C, There were no significant histone modifications (AcH3) around the β-actin promoter during memory consolidation (n = 3; one-way ANOVA, *p < 0.05, compared with naive controls; Student's t test, ^#p < 0.05, compared with context + shock alone). Error bars indicate SEM.