Figure 7.

Inhibition of NMDA receptor activation prevents bdnf DNA demethylation and mRNA expression. A, Diagram outlines the experimental design used with data presented in B–D. B, At 2 h after fear conditioning, NMDA receptor inactivation with MK801 prevented an increase in bdnf total mRNA (exon IX, t₍₉₎ = 4.18, p = 0.0024, n = 7–8). C, NMDA receptor inactivation prevented the demethylation of exon IV induced by fear conditioning (methylated exon IV, t₍₁₀₎ = 2.99, p = 0.0308; unmethylated exon IV, t₍₁₁₎ = 4.441, p = 0.1108, n = 6–7). D, NDMA receptor inactivation prevented changes in exon IV mRNA (exon IV, t₍₁₀₎ = 6.85, p = 0.0156, n = 6). The solid line across the bars represents normalized naive control levels (one-sample t test, *p < 0.05, **p < 0.01, ***p < 0.001, compared with naive controls; Student's t test, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, compared with context + shock). Error bars indicate SEM.