Table 1.

Auxin-responsive proteins identified in the microsomal fraction of rye coleoptiles treated for 2.0 h (± IAA; concentration: 10 μM). In the expression ratio column, positive numbers indicate up-regulated, and negative values denote down-regulated, proteins with respect to IAA treatment (average values of four independent experiments).

Spot	Protein name	Abund -ance ratio	P-value (t-test)	Gene locus	Number of unique peptides	Sequence coverage (%)	Best expected value
1	putative HSP70	− 1.34	0.0060	Bradi3g53100	22	21.0	2.5 e-7
2	alpha-glucosidase-like protein	− 1.35	0.0000011	Bradi1g69780	29	24.9	7.2 e-7
3*	phospholipase D	+ 1.53	0.0029	Bradi2g04480	12	18.1	1.1 e-8
	vacuolar proton- ATPase subunit A			Bradi1g31690	11	19.1	4.2 e-8
4	N-ethylmaleimide- sensitive fusion protein	− 1.25	0.0019	Bradi2g19570	28	−38.1	5.1e-8
5	N-ethylmaleimide- sensitive fusion protein	− 1.27	0.0064	Bradi2g19570	33	40.7	1.1e-8
6*	TCP-1/cpn60 chaperonin family	− 1.46	0.0021	Bradi5g02890	6	14.3	3.1e-8
	protein disulfide isomerase 1 pro-protein			Bradi4g23180	5	8.7	7.0 e-7
7*	Protein phosphatase 2A A subunit	− 1.46	0.00071	Bradi4g08720	7	17.5	6.7 e-8
	protein disulfide isomerase 1 pro-protein			Bradi4g23180	6	10.2	1.3e-7
8	dihydrolipoamide dehydrogenase	+ 1.59	0.0036	Bradi2g34770	40	70	3.0 e-8
9	dihydrolipoamide dehydrogenase	+ 1.30	0.0032	Bradi2g34770	44	69.2	1.2e-9
10	26S protease regulatory subunit T5	− 1.31	0.000024	Bradi3g54490	24	54.7	7.3e-9
11	26S protease regulatory subunit S10B	+ 1.33	0.010	Bradi1g36400	26	60.8	9.3e-9
12	26S protease regulatory subunit S10B	+ 1.48	0.018	Bradi1g36400	20	54.1	1.7e-8
13*	26S protease regulatory subunit S10B	− 1.46	0.00018	Bradi1g36400	11	33.2	1.1e-6
	large subunit ribosomal protein L4e			Bradi1g55510	7	24.4	3.9e-8
14	RNA helicase 2	+ 1.41	0.00050	Bradi3g60590	10	17.3	3.3e-6
15	putative protein	+ 1.32	0.017	Bradi2g43230	7	15.9	1.1e-5
16*	small Ras-related GTP- binding protein	− 1.33	0.0000077	Bradi2g15730	10	50.5	2.3e-6
	putative 40S ribosomal protein S3			Bradi3g16150	8	31.0	2.0e-9

^*Two proteins were identified in the same spots.