Fig. 3.

Effectiveness of B-cell depletion by anti-CD20 varies depending on the age when B cells are depleted. Flow cytometric analysis of B220+ B cells in the spleens of NOD.H-2h4 mice given isotype control or anti-CD20 10 and 16 days after birth (4 weeks groups) or at 8 weeks of age (10 weeks groups). Spleens were removed 14 days after injection of antibody and analyzed by flow cytometry. The results are representative of the groups shown in Table 2 and include six to eight individual mice assayed for each group.