Fig. 6.

T cells from isotype control-treated donors with SAT provide help to B cells from TCRα−/− mice, whereas T cells from anti-CD20-treated donors have minimal activity unless Treg are depleted. TCR-α−/− mice were irradiated (300 rad) and given 5 × 10⁶ splenic T cells (purified using nylon wool as described in Methods). T-cell donors were either naive NOD.H-2h4 mice or mice that were given the indicated treatment before receiving NaI water for 8 weeks (SAT donors). Recipients were given NaI in their water, and thyroids were removed 8 weeks later. Results are pooled from three separate experiments. Mice not given T cells did not develop SAT and their anti-MTg autoantibody responses were <0.100 OD units (data not shown). (a) SAT severity scores of individual mice 8 weeks after NaI water; the mean severity score for each group is indicated by the line. (b) Anti-MTg autoantibody responses expressed as mean OD₄₁₀ ± SEM of individual sera diluted 1/50. Groups differing significantly from the isotype control are indicated by the asterisk (P < 0.05).