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. 2012 Mar 26;3(3):53–60. doi: 10.4331/wjbc.v3.i3.53

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Interaction between IQGAP1 and CDC42. An interaction between IQGAP1(DR6) and CDC42 can be detected by EDC crosslinking in the presence of GTP. In the absence of EDC (lanes headed -EDC), a mixture of IQGAP(DR6) and CDC42 (lane C) behaves the same on SDS-PAGE as IQGAP1(DR6) and nucleotide-depleted CDC42 (lane ND) and as IQGAP1(DR6) and GTP-loaded CDC42 (lane GTP). In the presence of the crosslinker (lanes headed +EDC), crosslinking was not observed between IQGAP(DR6) and CDC42 (C) or IQGAP(DR6) and nucleotide-depleted CDC42 (ND). However an additional band, corresponding to approximately the combined molecular masses of IQGAP(DR6) and CDC42 is seen with GTP-loaded CDC42 (GTP).