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Interaction of IQGAP-CTD and CDC42 can be detected by surface plasmon resonance. Typical sensorgrams resulting from flowing IQGAP1(DR6) wild type and variants over immobilised CDC42 (for conditions, see Materials and Methods). From top to bottom, wild type, S1441E, S1443D, S1441E/S1443D, M1231I. Protein concentrations are shown to the right of the sensorgrams.
