FIG 1 .

Identification of the F. novicida phosphofructokinase gene. (A) Glycolysis pathway steps. F. novicida gene assignments are derived from the sequence annotation, and results are presented here. (B) Growth of mutants on different carbon sources. The gluconeogenesis mutant (gplX) is selectively defective for growth on short-chain carbon sources, whereas the aldolase mutant (fbaA), defective in both glycolysis and gluconeogenesis, is defective for growth on both sugars and short-chain carbon sources. Mutations in FTN_1210 lead to defects in growth on sugars, suggesting a glycolysis defect. NAG, N-acetylglucosamine. (C) Complementation of an E. coli phosphofructokinase mutant with FTN_1210. The pronounced growth defect of an E. coli pfk mutant (JW3887) on sorbitol as the carbon source (40) is complemented by expression of FTN_1210, supporting its assignment as the pfk gene. FTN_1210 also complemented an E. coli pfk mutant growth defect on glucose (not shown).