Figure 8.

The MEPAL output of the energy profile of the aquaporin-2 wild type and its two mutants D150E and G196D. The distance tree derived by MEPAL indicates the distinct similarity of the energy profiles of the mutants (a). This is visualized by the MEPAL output (b). In the regions, which surround the mutated residues, similarities in energy profile progression can be seen. Thus, it is supported that both mutations affect protein stability and the transcellular water flux as described in literature [45].