Table 2.
Cell distribution in different phases of cell cycle determined by FACS analysis.
| Clones | % fluorescence at cell cycle phase | ||
|---|---|---|---|
| G1 | S | G2 | |
| Control R2Zeo | 50.3 (0.2) | 23.6 (0.2) | 23.8 (0.1) |
| HA-CKI-γ2-Wild Type | |||
| Z6 | 67.6 (0.4)* | 12.3 (0.4)* | 15.3 (0.6)* |
| Z23 | 70.2 (0.3)* | 17.0 (0.2)* | 12.1 (0.4)* |
Cells were fixed with 70% ethanol, stained with propidium iodide, and subjected to flow cytometry analysis. Values are mean (SEM) of 4 assays. *P at least ≤ 0.00001 compared to control.