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. Author manuscript; available in PMC: 2012 Mar 26.
Published in final edited form as: Cell. 2011 Dec 23;147(7):1628–1639. doi: 10.1016/j.cell.2011.09.057

Figure 1. A systematic approach for associating CRs to genomic loci and chromatin states.

Figure 1

(A) CR associations with chromatin modification states. Right: The chromatin regulator EZH2 (pink gear) is associated (arrow) with a locus marked by the histone modification H3K27me3 (small red ball). Left: For many other chromatin regulators (non-colored gears, CR) their target loci and associated modifications (different colored balls) remain unknown. (B) Our process consists of four steps (left to right): 1. ChIP assays were performed with 145 different antibodies targeting 92 distinct CRs and 15 modifications. 2. ChIP samples were screened for enrichment across 487 signature loci by ChIP-string to identify promising CR antibodies. 3. These antibodies were applied in ChIP-seq to generate genome-wide maps for 29 CRs. 4. We identified target loci and associated histone modifications for each CR. We found that the CRs partition into six modules with correlated binding patterns, and also exhibit instances of combinatorial binding.