Table 2.
Comparison of methods to determine phylogenetic distribution of archaeal lineages in Norris Geyser Basin OSP Spring.
| Taxonomic lineage | Metagenome read binning (% total Archaea) | JGI ribosomal panel (% total Archaea) | 16S rRNA pyrotag sequences (% total Archaea) | Near full-length metagenome 16S rRNA sequences: Closest cultured relative or environmental sequence (% = nucleotide similarity; length bp) |
|---|---|---|---|---|
| OSPB | ||||
| NAG1 | 60.7 | 60.3 | 49.2 | Thermofilum sp. 1505; 84% Koz183; 100% (1492) |
| Metallosphaera | 11.7 | 16.1 | 21.3 | Metallosphaera yellowstonensis str. MK1; 100% (1278) |
| Thermoproteales | 15.2 | 9.9 | 12.6 | Vulcanisaeta distributa IC-017; 98.4% SK409; 99.9% (1151) |
| Acidilobus | 5.6 | 7.9 | 6.2 | None |
| NAG2 | 1.1 | 3.4 | 2.5 | Staphylothermus sp. 1633; 85.2% Koz169; 100% (1046) |
| Sulfolobales str. MK5 | nd | 0 | 2.4 | None |
| Thaumarchaeota | nd | 0 | 1.3 | None |
| Other Archaea | 5.8% unassigned contigs | 2.4 | 3.7 | None |
| OSPC | ||||
| NAG1 | 56.5 | 57.1 | Thermofilum sp. 1505; 84% Clone Koz183; 100% (1334) | |
| NAG2 | 28.7 | 17 | Staphylothermus sp. 1633; 85.7% YNPFFA85; 99.6% (1354) | |
| Metallosphaera | 9.9 | 12 | Metallosphaera yellowstonensis str. MK1; 99.6% (1354) | |
| Thermoproteales | nd | 9.2 | None | |
| Sulfolobales str. MK5 | nd | 1.5 | None | |
| Other archaea | 5% unassigned contigs | 3.1 | None | |
| OSPD | ||||
| NAG2 | 31.8 | 32 | Seq1: Staphylothermus hellenicus;85.7% YNPFFA4 99.6% (1401) Seq2: Staphylothermus sp.1633; 85.7% YNPFFA85; 99.6% (1358) | |
| NAG1 | 19.8 | 32 | Thermofilum sp. 1505; 84% Koz183; 100% (1334) | |
| Metallosphaera | 19.2 | 21.2 | Metallosphaera yellowstonensis str. MK1; 99.5% (1354) | |
| Sulfolobales str. MK5 | 13.3 | 8.5 | Sulfolobales str. MK5; 99.8% (1408) | |
| Novel Euryarchaeota | 8.1 | 0 | Aciduliprofundum sp. EPR07-39; 86.5 BSLdp48 (1297) | |
| Sulfolobus | 7.8 | 4.3 | None | |