Table 3.
Novel isolates obtained from acid-sulfate-chloride (ASC) springs and characterization of cell morphology, pH optima, temperature optima, and growth on iron and sulfur substrates as electron donors or Fe(III) as an electron acceptor under anaerobic conditions.
| Isolate | Cell morphology | Closest 16S rRNA relative (% similarity) | pHoptima | Toptima (°C) | Fix CO2 | Plate | H2 | Fe2+ | S0 | Pyrite | Fe3+ |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sulfolobus str. MK3 | ∼1 μm cocci | Sulfolobus sp. T1 (96.6%) | 2.0–2.5 | 75 | + | − | − | + | + | + | − |
| Sulfolobales str. MK5 | ∼1 μm cocci | Sulfolobus islandicus HVE10/4 (88%) | 2.0–2.5 | 60–70 | − | + | − | + | + | + | + |
| Sulfobacillus str. MK2 | ∼3–5 μm rods | Sulfobacillus acidophilus TPY (98.3%) | 2.0–3.0 | 55–58 | − | + | − | + | + | + | − |
| Acidicaldus str. MK6 | ∼1.2–1.5 μm rods up to 20–30 μm filaments | Acidicaldus organivorans str. Y008 (97.6%) | 2.5–3.0 | 55–60 | − | + | − | − | − | − | + |
The ability to grow on solid media is also indicated. nd, Not determined.