Figure 5.

Effects of organic nitrates on the human HO-1 promoter activity. DLD1-HO-11kb-Prom cells were treated for 8 h with isosorbide dinitrate ISDN, PETN, isosorbide-5-mononitrate ISMN, or GTN at a concentration of 50 μM or the respective solvent (DMSO, H₂O, EtOH). Extracts were prepared, and luciferase activity and protein content were measured, and luciferase activity was normalized to the protein content. Shown (mean ± SEM; n = 8–10) are the normalized luciferase activity values. The normalized luciferase activity of the cell treated with solvent was set to 100%. (***= P < 0.001, ns: not significant different from solvent-treated cells.) These data were partly published in [34].