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Different strategies to complex DNA with PLGA-based nanoparticles. (a) Structure of polylactic-co-glycolic acid (PLGA) and linear polyethylenimine (PEI). A branched PEI can also be utilized to form complexes. (b) Schematic of the preparation methods of PLGA formulations using methods that result in plasmid DNA incorporation on the inside or the surface of PLGA particles as reported in [3, 40].
